Sample-to-answer in-situ, 3D, single cell spatial analysis
Access the power of the Pyxa platform - the only technology that provides high-plex multiomics data from individual cells in thick tissue sections - with our end-to-end services.
Getting started is easy. Send Stellaromics your thick tissue samples, and we’ll handle the rest. Our process consists of:
- Tissue sectioning and placing tissue into the Pyxa well plate.
- Full sample preparation with target probe set of interest.
- In situ 3D sequencing on cleared tissue.
- Data analysis and report generation.
Click below to see our sample inputs and data outputs generated by our data analysis systems.
Inputs
- You provide an embedded tissue block. We handle the rest from sample to answer!
- Gene panel customization is available.
Outputs
- Cell by gene matrix including XYZ locations and subcellular localization.
- 3D cell mosaic and cell segmentation TIFF images.
- Customized data and QC reports.
- Guidance on open-source visualization and analysis tools.
Leverage our pre-validated mouse brain 3D pan-neural
cell typing panel for faster results
For neuroscience applications, the ready-to-use 250 Gene Mouse Brain Panel provides ample transcriptional coverage, regardless of the brain region, with an emphasis on genes that are functionally relevant for inflammation, recent neuronal activity, and more, including:
- Major neurotransmitter genes of interest: Slc17a6/7/8, Slc32a1, Gad1, Gad2, Th, Ddc, Slc6a3, Slc18a2, Chat, Tph2, and many neuropeptides.
- Inclusion of canonical markers neuroscientists rely on: cortical interneuron markers, steroid hormone receptors (Esr1, Ar, Pgr), Drd1/Drd2, other neuropeptides and receptors.
- Inclusion of general markers relevant for most experiments: activation of astrocytes and microglia (Gfap, Aldoc, Cd68, Cd45), IEGs (Fos, Egr1), cell division (Top2a).
Full gene list available by request.
Not working on a neuroscience project? Let us know about your project, and we can explore full sample preparation with customizable set of target probes.
